Multiple stresses on the oxidative metabolism of sugarcane varieties
The exposure of plants to multiple stress conditions can cause over production of reactive oxygen species promoting an imbalance in the redox system. The aim of this study was to examine the antioxidant response from varieties of sugarcane under adverse conditions, to understand the defense mechanis...
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Veröffentlicht in: | Ciência rural 2018-01, Vol.48 (4) |
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Zusammenfassung: | The exposure of plants to multiple stress conditions can cause over production of reactive oxygen species promoting an imbalance in the redox system. The aim of this study was to examine the antioxidant response from varieties of sugarcane under adverse conditions, to understand the defense mechanisms of tolerant and sensitive genotypes. Micropropagated sugarcane of six genotypes, RB966928, RB98710, RB855453, RB99395, RB867515, and RB855156, were subjected to isolated action of high-temperature stress and a combination of water and saline stress, and effects were evaluated. Activities of enzymes such as superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), as well as stress indicators such as lipid peroxidation (MDA content) and hydrogen peroxide (H2O2) content were evaluated. The RB867515 variety showed synchrony between the enzymatic activity and ROS regulation and detoxification, in the plant cell under environmental stress situations, thereby ensuring the maintenance of the relative water content without increment of lipid peroxidation; and consequently, the maintenance of physiological processes and plant growth. These results indicated that lipid peroxidation associated with relative water content can be good biochemical markers of sensitivity to multiple stresses in sugarcane.
RESUMO: A exposição de plantas a condições de estresse múltiplo pode causar uma superprodução de espécies reativas de oxigênio promovendo desequilíbrio no sistema redox. O objetivo deste trabalho foi estudar a resposta antioxidante de variedades de cana-de-açúcar sob condições adversas visando a compreensão de mecanismos de defesa de genótipos tolerantes e sensíveis. Mudas micropropagadas de cana-de-açúcar de seis genótipos, RB966928, RB98710, RB855453, RB99395, RB867515 e RB855156 foram submetidas à avaliação da ação isolada do estresse por alta temperatura e da sua combinação com o estresse hídrico e salino. Avaliou-se a atividade das enzimas superóxido dismutase (SOD), catalase (CAT) e ascorbato peroxidase (APX), bem como a peroxidação lipídica (teor de MDA) e teor de peróxido de hidrogênio (H2O2), indicadores de estresse. A variedade RB867515 destacou-se por apresentar sincronia entre a atividade enzimática na regulação e desintoxicação das ROS, produzidas na célula vegetal durante situações de estresse ambiental, garantindo a manutenção do teor relativo de água sem incremento da peroxidação lipídica e, consequentemente, a manutenção dos processos fis |
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ISSN: | 0103-8478 1678-4596 1678-4596 |
DOI: | 10.1590/0103-8478cr20141487 |