Asian Sand Dust Particles Enhance the Development of Aspergillus fumigatus Biofilm on Nasal Epithelial Cells

Asian sand dust (ASD) and are known risk factors for airway mucosal inflammatory diseases. Bacterial and fungal biofilms commonly coexist in chronic rhinosinusitis and fungus balls. We evaluated the effects of ASD on the development of biofilm formation on nasal epithelial cells. Primary nasal epithelial cells were cultured with conidia with or without ASD for 72 h. The production of interleukin (IL)-6, IL-8, and transforming growth factor (TGF)-β1 from nasal epithelial cells was determined by the enzyme-linked immunosorbent assay. The effects of ASD on biofilm formation were determined using crystal violet, concanavalin A, safranin staining, and confocal scanning laser microscopy. ASD and significantly enhanced the production of IL-6 and IL-8 from nasal epithelial cells. By coculturing with ASD, the dry weight and safranin staining of the fungal biofilms significantly increased in a time-dependent manner. However, the increased level of crystal violet and concanavalin A stain decreased after 72 h of incubation. ASD and induced the production of inflammatory chemical mediators from nasal epithelial cells. The exposure of to ASD enhanced the formation of biofilms. The coexistence of ASD and may increase the development of fungal biofilms and fungal inflammatory diseases in the sinonasal mucosa.