Effect of Nitrogen Sources on Omega-3 Polyunsaturated Fatty Acid Biosynthesis and Gene Expression in Thraustochytriidae sp

The molecular mechanism that contributes to nitrogen source dependent omega-3 polyunsaturated fatty acid (n-3 PUFA) synthesis in marine oleaginous protists sp., was explored in this study. The fatty acid (FA) synthesis was significantly influenced by the supplement of various levels of sodium nitrat...

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Veröffentlicht in:	Marine drugs 2020-12, Vol.18 (12), p.612
Hauptverfasser:	Li, Siting, Hu, Zhangli, Yang, Xuewei, Li, Yan
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyl-CoA carboxylase Biomass Biosynthesis Cell growth Cultivation Docosahexaenoic acid elongation and unsaturation Enoyl-CoA hydratase Fatty acids Fermentation Folic acid Gene expression Gene regulation Genes Lipids Metabolism Mitochondria n-3 polyunsaturated fatty acids Nitrates Nitrogen nitrogen source Nitrogen sources Nucleolin Nucleotide sequence PCR Polymerase chain reaction Polyunsaturated fatty acids Protists reference genes Sodium Sodium nitrate Statistical methods Thraustochytriidae sp Trends Urea
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Zusammenfassung:	The molecular mechanism that contributes to nitrogen source dependent omega-3 polyunsaturated fatty acid (n-3 PUFA) synthesis in marine oleaginous protists sp., was explored in this study. The fatty acid (FA) synthesis was significantly influenced by the supplement of various levels of sodium nitrate (SN) (1-50 mM) or urea (1-50 mM). Compared with SN (50 mM) cultivation, cells from urea (50 mM) cultivation accumulated 1.16-fold more n-3 PUFAs (49.49% docosahexaenoic acid (DHA) ( / , of total FAs) and 5.28% docosapentaenoic acid (DPA) ( / , of total FAs)). Strikingly higher quantities of short chain FAs (
ISSN:	1660-3397 1660-3397
DOI:	10.3390/md18120612