Effect of two organic fertilizers on some anatomical features of Pinus pinea L. seedlings grown in field and lathe house

Pinus pinea L. cultivated in open field and lath house treated with 0, 12g. plant-1 local compost and 9 g.plant-1 Bamboo biochar was studied by using Factorial Complete Randomized Design and carried out during 4th October-2020 to 5th May-2021. Cultivation of the seedlings in open field without fertilizers gave best values of epidermis, mesophyll and vascular bundle thickness (11.333, 39.000 and 55.000um respectively), the highest value of hypoderm thickness (14.000um) was recorded from combination of open field condition and Bamboo biochare fertilizer. While, the best stomata density was with application of Bamboo biochar under lath house condition (113.667.mm-2). Epidermis thickness (23.667 um) was obtained from the treatment of 12(g. plant-1) local compost and lath house condition. However, the treatment of 12g. Plant-1 local compost gave the best vascular bundle thickness (176.667um) and the beast result of cortex thickness (91.000 um) was in the treatment of open field combined with 9 g.plant-1 Bamboo biochar. References 1-Luomala, E.M., Laitinen, K., Sutinen, S., Kellomäki, S. and Vapaavuori, E., 2005. Stomatal density, anatomy and nutrient concentrations of Scots pine needles are affected by elevated CO2 and temperature. Plant, Cell & Environment, 28(6), pp.733-749. 2-Kilpeläinen, A., Gerendiain, A.Z., Luostarinen, K., Peltola, H. and Kellomäki, S., 2007. Elevated temperature and CO2 concentration effects on xylem anatomy of Scots pine. Tree Physiology, 27(9), pp.1329-1338. 3-Sun, J., He, F., Shao, H., Zhang, Z. and Xu, G., 2016. Effects of biochar application on Suaeda salsa growth and saline soil properties. Environmental Earth Sciences, 75(8), p.630. 4- Muñiz, G.I.B.D., Lengowski, E.C., Nisgoski, S., Magalhães, W.L.E.D., Oliveira, V.T.D. and Hansel, F., 2014. Characterization of Pinus spp needles and evaluation of their potential use for energy. Cerne, 20, pp.245-250. 5-Galdina, T. and Khazova, E., 2019, August. Adaptability of Pinus sylvestris L. to various environmental conditions. In IOP Conference Series: Earth and Environmental Science (Vol. 316, No. 1, p. 012002). IOP Publishing. 6- Gebauer, R., Volařík, D., Urban, J., Børja, I., Nagy, N.E., Eldhuset, T.D. and Krokene, P., 2015. Effects of prolonged drought on the anatomy of sun and shade needles in young Norway spruce trees. Ecology and evolution, 5(21), pp.4989-4998. 7- Dörken, V.M. and Lepetit, B., 2018. Morpho‐anatomical and physiological differences between sun and shade lea