Effect of Three Different Centrifugation on Cryosurvival of Goat Spermatozoa Supplemented with Vitamin E

Three different centrifugation frequencies viz., zero time (ZTC), one time (ITW), two times (2TC) and three times (3TC) supplemented with different levels of vitamin E were studied under in vitro cryosurvival of West African Dwarf (WAD) goat spermatozoa. Semen samples were washed ITW, 2TC and 3TC and supplemented each with 2, 4, 6 and 8 mM of vitamin E in a 3x4 factorial arrangements and cryopreserved for 30 days in liquid nitrogen. Data were subjected to one-way ANOVA. Spermatozoa cryopreserved with Tris-based extender supplemented with 6 and 8 mM using 3TC and 8 mM with 2TC had highest (P