Structural characterization, antioxidant and cytotoxic effects of iron nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract

was used to synthesize FeNP . They were characterized by UV-vis spectroscopy, FT-IR, TEM, SEM, XRD and ZP. The aim of this study was to evaluate in vitro cytotoxic activity and antioxidant acitivities of FeNP and . The antioxidant properties were evaluated using DPPH, ABTS and H assays. FeNP had higher antioxidant activity comparing to according to DPPH (IC : 3.48 μg/mL) and ABTS (60.52%) assays. Anti-cancer activities of FeNP and were investigated in breast cancer, melanoma and control cell lines. FeNP was more cytotoxic than in MCF-7, MeWo, CHL-1, and HEL 299 cells. FeNP had shown that mitochondria induce apoptosis through stress in MDA-MB-231, and cells MeWo. also induced apoptosis 2.23-fold in MCF-7 cells. Progesterone receptor gene expression showed a 10-fold increase in a hormone-dependent MCF-7 cell line in , and FeNP treatment. Expressions of BCL6, CXCL12, DNAJC15, RB1 and TPM1 in melanoma cancer cell lines were significantly increased in and FeNP administration. It had been shown that FeNP regulates gene expressions that may be considered important in terms of prognosis in breast cancer and melanoma cell lines and it is suggested that gene expressions regulated by FeNP are also evaluated in animal models in vivo.