In vitro biofilm formation by Staphylococcus aureus isolated from wounds of hospital-admitted patients and their association with antimicrobial resistance

including methicillin-resistant (MRSA) has the propensity to form biofilms, and causes significant mortality and morbidity in the patients with wounds. Our aim was to study the in vitro biofilm-forming ability of isolated from wounds of hospitalized patients and their association with antimicrobial resistance. Forty-three clinical isolates of were obtained from 150 pus samples using standard microbiological techniques. Biofilm formation in these isolates was detected by tissue culture plate (TCP) method and tube adherence method (TM). Antimicrobial susceptibility test was performed using the modified Kirby-Bauer disk diffusion method as per Clinical and Laboratory Standards Institute guidelines. MRSA was detected using the cefoxitin disk test. Biofilm formation was observed in 30 (69.8%) and 28 (65.1%) isolates of via TCP method and TM, respectively. Biofilm-producing exhibited a higher incidence of antimicrobial resistance when compared with the biofilm nonproducers (