Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms

This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or ca...

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Veröffentlicht in:Cells (Basel, Switzerland) Switzerland), 2020-11, Vol.9 (12), p.2546
Hauptverfasser: Blanco-Prieto, Olga, Catalán, Jaime, Trujillo-Rojas, Lina, Peña, Alejandro, Rivera Del Álamo, Maria Montserrat, Llavanera, Marc, Bonet, Sergi, Fernández-Novell, Josep Maria, Yeste, Marc, Rodríguez-Gil, Joan E
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Sprache:eng
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Zusammenfassung:This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. Whereas exposure for 1 and 5 min significantly ( < 0.05) decreased total motility and intracellular ATP levels, irradiation for 10 min induced the opposite effect. Oligomycin A abolished the light-effects on intracellular ATP levels, O consumption and mitochondrial membrane potential, whereas compared to non-irradiated samples, FCCP significantly ( < 0.05) increased O consumption when sperm were irradiated for 1 min. Both oligomycin A and FCCP significantly ( < 0.05) decreased total motility. Red-light increased cytochrome oxidase activity with a maximal effect after 5 min of irradiation, which was abolished by both oligomycin A and FCCP. In conclusion, red-light modulates sperm mitochondrial function via electron chain activity in an exposition, time-dependent manner.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells9122546