Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells

Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells

Objective To investigate the effect of sanguinarine on the invasion and migration of lung adenocarcinoma cells and its preliminary molecular mechanism. Methods MTT assay was used to observe the proliferative capacity of A549 and H1975 cells. Wound healing and Transwell assays were performed to deter...

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Veröffentlicht in:Zhongliu fangzhi yanjiu 2019-12, Vol.46 (12), p.1057-1061
Hauptverfasser: Yang, Jia, Chen, Min, Li, Minghua, Qin, Jingru, Wang, Zhongqi
Format: Artikel
Sprache:chi
Schlagworte:
Adenocarcinoma
Cell migration
Dishevelled protein
Gene expression
invasion
LEF protein
lung adenocarcinoma
Lung cancer
Lungs
migration
mRNA
Protein expression
Proteins
Sanguinarine
Signal transduction
Signaling
Time dependence
Wnt protein
wnt/β-catenin signaling pathway
Wound healing
β-Catenin
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Zusammenfassung:Objective To investigate the effect of sanguinarine on the invasion and migration of lung adenocarcinoma cells and its preliminary molecular mechanism. Methods MTT assay was used to observe the proliferative capacity of A549 and H1975 cells. Wound healing and Transwell assays were performed to determine the invasion and migration abilities of A549 and H1975 cells, respectively. RTqPCR and Western blot were used to detect the mRNA and protein expression of core genes in Wnt/β-catenin signaling pathway. Results Compared with the control group, sanguinarine(1-10μmol/L) could inhibit the activity of human lung adenocarcinoma cells in a concentration- and time- dependent manner (P
ISSN:1000-8578
1000-8578
DOI:10.3971/j.issn.1000-8578.2019.18.1576