A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains

A convenient and effective sucrose transport assay for strains is needed. Traditional methods, such as C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into , and a fluorescence assay based on esculin was used for the analysis of sucrose transport in strains. This showed that the heterologously expressed potato sucrose transporter is functional in . Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in .