Pelagibaca bermudensis promotes biofuel competence of Tetraselmis striata in a broad range of abiotic stressors: dynamics of quorum-sensing precursors and strategic improvement in lipid productivity

Amelioration of biofuel feedstock of microalgae using sustainable means through synthetic ecology is a promising strategy. The co-cultivation model ( and ) was evaluated for the robust biofuel production under varying stressors as well as with the selected two-stage cultivation modes. In addition, t...

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Veröffentlicht in:Biotechnology for biofuels 2018-04, Vol.11 (1), p.102-102, Article 102
Hauptverfasser: Patidar, Shailesh Kumar, Kim, Sae-Hee, Kim, Jin Ho, Park, Jungsoo, Park, Bum Soo, Han, Myung-Soo
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Sprache:eng
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Zusammenfassung:Amelioration of biofuel feedstock of microalgae using sustainable means through synthetic ecology is a promising strategy. The co-cultivation model ( and ) was evaluated for the robust biofuel production under varying stressors as well as with the selected two-stage cultivation modes. In addition, the role of metabolic exudates including the quorum-sensing precursors was assessed. The co-cultivation model innovated in this study supported the biomass production of in a saline/marine medium at a broad range of pH, salinity, and temperature/light conditions, as well as nutrient limitation with a growth promotion of 1.2-3.6-fold. Hence, this developed model could contribute to abiotic stress mitigation of . The quorum-sensing precursor dynamics of the growth promoting bacteria exhibited unique pattern under varying stressors as revealed through targeted metabolomics (using liquid chromatography-mass spectrometry, LC-MS). and its metabolic exudates mutually promoted the growth of , which elevated the lipid productivity. Interestingly, hydroxy alkyl quinolones independently showed growth inhibition of on elevated concentration. Among two-stage cultivation modes (low pH, elevated salinity, and nitrate limitation), specifically, nitrate limitation induced a 1.5 times higher lipid content (30-31%) than control in both axenic and co-cultivated conditions. is established as a potential growth promoting native phycospheric bacteria for robust biomass generation of in varying environment, and two-stage cultivation using nitrate limitation strategically maximized the biofuel precursors for both axenic and co-cultivation conditions (T and T-PB, respectively). Optimum metabolic exudate of which act as a growth substrate to surpasses the antagonistic effect of excessive hydroxy alkyl quinolones [HHQ, 4-hydroxy-2-alkylquinolines and PQS (pseudomonas quorum signal), 2-heptyl-3-hydroxy-4(1H)-quinolone].
ISSN:1754-6834
1754-6834
DOI:10.1186/s13068-018-1097-9