Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry

To study the source of different gelatins of donkey-hide gelatin lumps, four kinds of donkey-hide gelatin lumps were added with low content (1 mg/mL) of bovine gelatin and porcine gelatin respectively. High-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) were chosen to identify the...

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Veröffentlicht in:Shipin gongye ke-ji 2022-02, Vol.43 (4), p.97-104
Hauptverfasser: Xiaomei SHA, Lijun ZHANG, Wenli JIANG, Guangyao WANG, Zuohua XIE, Guotai HUANG, Zongcai TU
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Sprache:chi
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Zusammenfassung:To study the source of different gelatins of donkey-hide gelatin lumps, four kinds of donkey-hide gelatin lumps were added with low content (1 mg/mL) of bovine gelatin and porcine gelatin respectively. High-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) were chosen to identify the characteristic peptides of donkey-hide gelatin, bovine gelatin and porcine gelatin. The results showed that 18, 3 and 5 common characteristic peptides were accurately detected in the four kinds of donkey-hide gelatin lump for donkey-hide gelatin, bovine gelatin and porcine gelatin, respectively, with 10, 1 and 1 from the α1 chain and 8, 2 and 4 from the α2 chain. Meanwhile, the number of common characteristic peptides with hydroxylated modifications was 15, 2 and 4 respectively, while those without any modification groups were 3, 1 and 1. These common characteristic peptides would provide a basis for the HPLC-MS/MS traceable identification of the gelatin source in donkey-hide gelatin lump, which is of reference sig
ISSN:1002-0306
DOI:10.13386/j.issn1002-0306.2021060180