Analysis of Phylogeny and Genetic Diversity of Endangered Romanian Grey Steppe Cattle Breed, a Reservoir of Valuable Genes to Preserve Biodiversity

Since 2000, the Food and Agriculture Organization of the United Nations (FAO) has been drawing attention to the increasing numerical decline of Podolian cattle, which include the Romanian Grey Steppe. Currently, this breed is endangered, numbering under 100 heads across the territory of the entire country. Due to its qualities of rusticity, adaptability, and increased resistance to diseases and severe climate conditions, the Grey Steppe is considered a valuable genetic reserve for improving livestock production. This study aimed to quantify the genetic diversity of a population of 32 cattle from the area of N-E Moldova through the analysis of two mitochondrial markers, cytochrome b and the d-loop, which have been proven to be relevant to studies of genetic diversity and phylogeny. The results obtained based on the statistical analysis of the data using nucleotide sequence analysis software (DnaSP, SeaView, MegaX, PopArt, etc.) demonstrated that the breed belonged to the ancestral P′QT haplogroup, with direct descent from Bos taurus primigenius. Within this haplogroup, five cattle were identified, which could be used in the selection of crosses, with the aim of preserving valuable genetic resources for the improvement of other cattle breeds and the protection of biodiversity.