MicroRNAs Expression Induces Apoptosis of Macrophages in Response to Leishmania major (MRHO/IR/75/ER): An In-Vitro and In-Vivo Study

We aimed to investigate the effect of miR-15a mimic and inhibitor of miR-155 expression on apoptosis induction in macrophages infected with Iranian strain of in-vitro and in-vivo. RAW 264.7 cells were infected with promastigotes (MRHO/IR/75/ER), and then were treated with miRNAs. For in-vivo experim...

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Veröffentlicht in:Iranian journal of parasitology 2020-10, Vol.15 (4), p.475-487
Hauptverfasser: Gholamrezaei, Mostafa, Rouhani, Soheila, Mohebali, Mehdi, Mohammadi-Yeganeh, Samira, Haji Molla Hoseini, Mostafa, Haghighi, Ali, Lasjerdi, Zohreh, Hamidi, Faezeh, Kazem Sharifi-Yazdi, Mohammad
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Sprache:eng
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Zusammenfassung:We aimed to investigate the effect of miR-15a mimic and inhibitor of miR-155 expression on apoptosis induction in macrophages infected with Iranian strain of in-vitro and in-vivo. RAW 264.7 cells were infected with promastigotes (MRHO/IR/75/ER), and then were treated with miRNAs. For in-vivo experiment, BALB/c mice were inoculated with promastigotes, and then they were treated with miRNAs. For evaluation of miRNA therapeutic effect, in-vitro and in-vivo studies were performed using quantitative Real-time PCR, Flow cytometry, lesion size measurement, and Limiting Dilution Assay (LDA). This study was performed in Shahid Beheshti University of Medical Sciences in 2019. In-vitro results of flow cytometry showed that using miR-15a mimic, miR-155 inhibitor or both of them increased apoptosis of macrophages. In in-vivo, size of lesion increased during experiment in control groups (
ISSN:1735-7020
2008-238X
DOI:10.18502/ijpa.v15i4.4851