Cloning and expression analysis of FeR2R3-MYB of anthocyanin synthesis-related genes of common buckwheat

MYB is a common transcription factors widely involved in the regulation of anthocyanidin biosynthesis. In order to explore the regulatory role of MYB transcription factors in the biosynthesis of common buckwheat anthocyanidins, we screened and cloned a MYB gene associated with anthocyanin biosynthesis from the transcriptomic data of common buckwheat varieties of safflower common buckwheat and Beizaosheng, and named it FeR2R3-MYB, GenBank login number was MT151381.1. The sequence was analyzed by bioinformatics analysis and qRT-PCR was used to analyze the expression characteristics of FeR2R3-MYB gene in Beizaosheng and safflower common buckwheat. The results were as follows: (1) FeR2R3-MYB gene was 831 bp in total length, encoding 276 amino acids. The relative molecular mass of the protein was 30.95 kD, the theoretical isoelectric point (pI) was 8.73, and the instability index of the protein was 69.64, which belonged to the unstable protein. The total hydrophobic value was -0.679, and the whole peptide chain sh