Clonal relatedness of coagulase-positive staphylococci among healthy dogs and dog-owners in Spain. Detection of multidrug-resistant-MSSA-CC398 and novel linezolid-resistant-MRSA-CC5

Nasal carriage of coagulase-positive staphylococci (CoPS) in healthy dogs could indicate increased risks of colonization for in-contact people or vice versa. This study determined the nasal carriage rate of CoPS among healthy dogs and in-contact people, their genotypic characteristics and phylogenetic relatedness. Nasal samples were collected from 27 households (34 dogs and 41 humans) in Spain. Staphylococci were identified by MALDI-TOF-MS, their antimicrobial resistance (AMR) genes and -types were tested by PCR/sequencing. The relatedness of CoPS from the same households was assessed by core genome single nucleotide polymorphisms (SNPs) analyses. carriage was found in 34.1% of humans (including one methicillin-resistant MRSA-CC5-t2220-SCC type-IV2B) and 5.9% of dogs; in 2.4% of humans and 32.4% of dogs; while was only detected in dogs (5.4%). Remarkably, one human co-carried / , while a dog co-carried the three CoPS species. Household density was significantly associated with carriage in households with > than 1 dog and >than 1 human (OR = 18.10, 95% CI: 1.24-260.93, = 0.034). Closely related (