Derivation of the human embryonic stem cell line RCe011-A (RC-7)

The human embryonic stem cell line RCe011-A (RC-7) was derived from a failed to fertilise oocyte voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The ce...

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Veröffentlicht in:	Stem cell research 2016-03, Vol.16 (2), p.485-488
Hauptverfasser:	De Sousa, P.A., Tye, B.J., Collins, D.M., Bruce, K., Dand, P., Russell, G., Bradburn, H., Downie, J.M., Bateman, M., Courtney, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Cell Differentiation Cells, Cultured Embryo, Mammalian - cytology Embryoid Bodies - cytology Genotype Histocompatibility Testing Human Embryonic Stem Cells - cytology Human Embryonic Stem Cells - metabolism Humans Karyotype Lewis X Antigen - metabolism Male Microsatellite Repeats - genetics Microscopy, Fluorescence Octamer Transcription Factor-3 - metabolism Polymerase Chain Reaction
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Beschreibung
Zusammenfassung:	The human embryonic stem cell line RCe011-A (RC-7) was derived from a failed to fertilise oocyte voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XY male karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
ISSN:	1873-5061 1876-7753
DOI:	10.1016/j.scr.2016.02.036