Analysis of aflatoxins B1 and G1 in maize by quechers

A reliable and easy method has been developed for the determination of aflatoxins B1 and G2 in maize samples. High performance liquid chromatography coupled with FLD (HPLC-FLD) with photochemical derivatization was used. Mycotoxins were extracted from maize using a QuEChERS-based extraction procedure. The optimized analytical conditions were evaluated in terms of recoveries, reproducibility, LOD, LOQ and linearity for aflatoxin B1 and aflatoxin G1 in maize. Extraction, chromatographic and detection conditions were optimized in order to increase sample sensitivity. The linearity was analyzed in the range of 0.4-20 ?g/kg and the correlation coefficients (R2) were higher than 0.99 for aflatoxins B1 and G1. Blank samples were spiked at 1.0, 2.0 and 4.0 ?g/kg, and the average recovery for aflatoxin G1 was 96.96?1.72% and for aflatoxin B1 it was 86.80?1.24%. RSDs were lower than 25% for both mycotoxins. LOD for both aflatoxins was 0.5 ?g/kg and LOQ was 1.0 ?g/kg, respectively. Razvijen je pouzdan i lak metod odredjivanja aflatoksina B1 i G1 u uzorcima kukuruza. Koriscene su visoko performantna tecna hromatografija uz FLD (HPLC-FLD) i fotohemijska derivatizacija. Mikotoksini su ekstrahovani iz kukuruza upotrebom QuEChERS ekstrakcione procedure. Optimizacija analitickih uslova je procenjena na nivou prinosa ekstrakcije, reproduktivnosti, LOD, LOQ i linearnosti aflatoksina B1 i aflatoksina G1 u kukuruzu. Ekstrakcija, hromatografija i uslovi odredjivanja su optimizovani u cilju povecanja osetljivosti. Linearnost je odredjena za opseg koncentracija od 0.4-20 ?g/kg sa koeficijentom korelacije (R2) visim od 0.99 za aflatoksine B1 i G1. Kontrolni uzorci su obogaceni na nivou 1.0, 2.0 i 4.0 ?g/kg, sa prosecnim vrednostima pronosa ekstrakcije od 96.96?1.72% za aflatoksin G1 i 86.80?1.24% za aflatoksin B1. RSD vrednosti su bile nize od 25% za oba mikotoksina. LOD oba aflatoksina je iznosio 0.5 ?g/kg sa LOQ od 1.0 ?g/kg.