A GMMA-CPS-Based Vaccine for Non-Typhoidal Salmonella

Non-typhoidal are a major cause of gastroenteritis worldwide, as well as causing bloodstream infections in sub-Saharan Africa with a high fatality rate. No vaccine is currently available for human use. Current vaccine development strategies are focused on capsular polysaccharides (CPS) present on the surface of non-typhoidal . This study aimed to boost the amount of CPS purified from for immunization trials. Random mutagenesis with Tn transposon increased the production of CPS colanic acid, by 10-fold compared to wildtype. Immunization with colanic acid or colanic acid conjugated to truncated glycoprotein D or inactivated diphtheria toxin did not induce a protective immune response in mice. However, immunization with Generalized Modules for Membrane Antigens (GMMAs) isolated from colanic acid overproducing isolates reduced colonization in mice. Our results support the development of a GMMA-CPS-based vaccine against non-typhoidal .