OsG6PGH1 affects various grain quality traits and participates in the salt stress response of rice

Cytoplasmic 6-phosphogluconate dehydrogenase (G6PGH) is a key enzyme in the pentose phosphate pathway that is involved in regulating various biological processes such as material metabolism, and growth and development in plants. However, it was unclear if affected rice grain quality traits. We perform yeast one-hybrid experiments and reveal that may interact with . Subsequently, yeast point-to-point experiments and local surface plasmon resonance experiments verified that can bind to . in rice is a constitutive expressed gene that may be localized in the cytoplasm. and protein-synthesis metabolism-related genes are significantly upregulated in overexpressing transgenic positive endosperm, corresponding to a significant increase in the number of protein bodies II, promoting accumulation of related storage proteins, a significant increase in grain protein content (GPC), and improved rice nutritional quality. positively regulates amylose content, negatively regulates chalkiness rate and taste value, significantly affects grain quality traits such as appearance, cooking, and eating qualities of rice, and is involved in regulating the expression of salt stress related genes, thereby enhancing the salt-stress tolerance of rice. Therefore, represents an important genetic resource to assist in the design of high-quality and multi-resistant rice varieties.