Carboxylicivirga marinus sp. nov., Isolated From Marine Sediment, and Genome Insight of the Genus Carboxylicivirga

A Gram-stain-negative, facultatively aerobic, beige and slender bacterium, designed N1Y132 T , was isolated from a sediment sample taken from coastal zone of Weihai, China (37°34′31.44″ N, 122°9′15″ E). Growth occurred at 15-33°C (optimal 25–28°C), at pH 6.5–8.5 (optimal pH 7.5), in 0–9% (w/v) NaCl (optimal 2.0–2.5%). According to the phylogenetic analysis based on the 16S rRNA gene sequences, strain N1Y132 T showed the highest sequence similarity (97.9%) with Carboxylicivirga taeanensis JCM 19490 T , followed by C. sediminis JR1 T (97.4%), C. mesophila JCM 18290 T (96.4%) and C. linearis FB218 T (95.1%). Thus, strain N1Y132 T was a member of the genus Carboxylicivirga. The average nucleotide identity (ANI) values between strain N1Y132 T and C. sediminis JR1 T , C. linearis FB218 T and C. mesophila JCM 18290 T were 74.8%, 71.1%, and 74.9%, respectively. Chemotaxonomic analysis showed that the sole respiratory quinone was MK-7 and the major fatty acids (> 5.0%) included iso-C 15 : 0 , anteiso-C 15 : 0 , iso-C 13 : 0 , iso-C 14 : 0 , iso-C 15 : 0 3-OH, and C 15 : 1 ω6 c . The polar lipids were consisted of a phosphatidylethanolamine, two phosphoaminolipids and six unidentified lipids. The DNA G + C content was 38.1 mol%. Based on the evidence presented in this study, strain N1Y132 T represents a novel species of the genus Carboxylicivirga , for which the name Carboxylicivirga marinus sp. nov. is proposed. The type strain is N1Y132 T (= KCTC 72934 T = MCCC 1H00431 T ).