Nasal DNA methylation at three CpG sites predicts childhood allergic disease

Childhood allergic diseases, including asthma, rhinitis and eczema, are prevalent conditions that share strong genetic and environmental components. Diagnosis relies on clinical history and measurements of allergen-specific IgE. We hypothesize that a multi-omics model could accurately diagnose child...

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Veröffentlicht in:Nature communications 2022-12, Vol.13 (1), p.7415-13, Article 7415
Hauptverfasser: van Breugel, Merlijn, Qi, Cancan, Xu, Zhongli, Pedersen, Casper-Emil T., Petoukhov, Ilya, Vonk, Judith M., Gehring, Ulrike, Berg, Marijn, Bügel, Marnix, Carpaij, Orestes A., Forno, Erick, Morin, Andréanne, Eliasen, Anders U., Jiang, Yale, van den Berge, Maarten, Nawijn, Martijn C., Li, Yang, Chen, Wei, Bont, Louis J., Bønnelykke, Klaus, Celedón, Juan C., Koppelman, Gerard H., Xu, Cheng-Jian
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Sprache:eng
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Zusammenfassung:Childhood allergic diseases, including asthma, rhinitis and eczema, are prevalent conditions that share strong genetic and environmental components. Diagnosis relies on clinical history and measurements of allergen-specific IgE. We hypothesize that a multi-omics model could accurately diagnose childhood allergic disease. We show that nasal DNA methylation has the strongest predictive power to diagnose childhood allergy, surpassing blood DNA methylation, genetic risk scores, and environmental factors. DNA methylation at only three nasal CpG sites classifies allergic disease in Dutch children aged 16 years well, with an area under the curve (AUC) of 0.86. This is replicated in Puerto Rican children aged 9–20 years (AUC 0.82). DNA methylation at these CpGs additionally detects allergic multimorbidity and symptomatic IgE sensitization. Using nasal single-cell RNA-sequencing data, these three CpGs associate with influx of T cells and macrophages that contribute to allergic inflammation. Our study suggests the potential of methylation-based allergy diagnosis. Accurate prediction of the onset of childhood allergy is important to clarify the difference between various respiratory diseases. Here the authors propose that the methylation status of three sites in nasal DNA predicts the onset of childhood allergy which may aid diagnosis and monitoring.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-022-35088-6