Optimization of a Simplified and Effective Analytical Method of Pesticide Residues in Mealworms ( Tenebrio molitor Larvae) Combined with GC-MS/MS and LC-MS/MS

An effective analytical method was optimized for residues including chlorpyrifos-methyl, deltamethrin, fenoxanil, thiobencarb and fludioxonil in mealworms, the larval form of . They are listed for pest control during wheat cultivation and can be found in wheat-bran feed for growing mealworms in South Korea. Analytes were extracted using acetonitrile and salt packet. Four clean-up methods ((1) MgSO + 25 mg PSA + 25 mg C18; (2) MgSO + 50 mg PSA + 50 mg C18; (3) EMR-lipid tube; and (4) 10 mL -hexane) were investigated and the method (1) was selected due to its robustness. Low-temperature precipitation of fat and proteins improved the recoveries. Recoveries from the Method (1) were satisfying with 70-120% with