Performance and acceptability of self-collected human papillomavirus testing among women living with HIV

•Women living with HIV are under-screened for cervical cancer.•Human papillomavirus (HPV) self-testing at home could become a more efficient strategy in the post-COVID-19 era.•Self-collected HPV testing has good sensitivity and moderate specificity among women living with HIV.•Self-sampling is highly accepted by women.•Improvement in self-sampling techniques is needed due to possibly inadequate sample volume. To assess the validity, reliability, and acceptability of self-collected human papillomavirus (HPV) tests in women living with HIV (WLHIV) in the United States. WLHIV ≥30 years of age underwent self-collected (clinic and home) and clinician-collected HPV tests. Sensitivity and specificity analyses were performed using the clinician-collected HPV tests as the comparator. The unweighted kappa statistic was used to evaluate the validity and reliability of self-collected HPV testing, and the level of agreement between the clinician-collected mRNA test and a DNA test that was used for routine care. A 13-question survey was used to assess acceptability. Among the 70 participants, the median age was 50 years, 75% had an undetectable HIV RNA, and 11% had a CD4 count of