Application Of Hydroxyapatite In Protein Purification

The precursors, Na2HPO4.2H2O and CaCl2.2H2O are used for synthesizing pure hydroxyapatite which less of carbonatecontent. The high temperature of sintering, about 700oC of temperature, is treated to minimize the carbonate group onhydroxyapatite surface. Carbonate content of hydroxyapatite which is sintered in 700oC is less than 110oC. It indicatesan increasing temperature of sintering will increase crystallinity and decrease carbonate content of hydroxapatite. Thismethod gave better way to resulting hydroxyapatite crystal. The patterns of band of SDS PAGE in resulting of ProteinPurification by DEAE matrix is also appear in using hydroxyapatite matrix. The other band also appear in purificationby hydroxyapatite matrix, it showed that hydroxyapatite not only has DEAE matrix characteristic as anion exchange butalso has kation exchange characteristic. These patterns proof that hydroxyapatite could be used in protein purificationas matrix as cation an anion exchange . Additionally, apatite matrix is abiding and can be used repeatedly more than onehundred times without contamination.