Phenotypic characterization and analysis of complete genomes of two distinct strains of the proposed species “L. swaminathanii”

Recently, a new Listeria species, “ Listeria swaminathanii ”, was proposed. Here, we phenotypically and genotypically characterize two additional strains that were previously obtained from soil samples and compare the results to the type strain. Complete genomes for both strains were assembled from...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Scientific reports 2022-06, Vol.12 (1), p.9137-9137, Article 9137
Hauptverfasser: Hudson, Lauren K., Chaggar, Harleen K., Schamp, Claire N., Claxton, Michelle L., Bryan, Daniel W., Peters, Tracey L., Song, Yaxiong, Carlin, Catharine R., den Bakker, Henk C., Denes, Thomas G.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Recently, a new Listeria species, “ Listeria swaminathanii ”, was proposed. Here, we phenotypically and genotypically characterize two additional strains that were previously obtained from soil samples and compare the results to the type strain. Complete genomes for both strains were assembled from hybrid Illumina and Nanopore sequencing reads and annotated. Further genomic analysis including average nucleotide identity (ANI) and detection of mobile genetic elements and genes of interest (e.g., virulence-associated) were conducted. The strains showed 98.7–98.8% ANI with the type strain. The UTK C1-0015 genome contained a partial monocin locus and a plasmid, while the UTK C1-0024 genome contained a full monocin locus and a prophage. Phenotypic characterization consistent with those performed on the proposed type strain was conducted to assess consistency of phenotypes across a greater diversity of the proposed species (n = 3 instead of n = 1). Only a few findings were notably different from those of the type strain, such as catalase activity, glycerol metabolism, starch metabolism, and growth at 41 °C. This study further expands our understanding of this newly proposed sensu stricto Listeria species.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-13119-y