Determination of the ELISA kits for screening convalescence plasma with high potency of SARS-CoV-2 IgG
Objective To determine the ELISA kit for screening convalescence plasma with high potency of SARS-CoV-2 IgG by comparing and analyzing the plasma detection results of convalescent plasma collected in different periods via ELISA kits from two manufacturers and the results of mixed plasma with differe...
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Veröffentlicht in: | Zhongguo shu xue za zhi 2023-03, Vol.36 (3), p.199-203 |
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Hauptverfasser: | , , , , , , , , , , , |
Format: | Artikel |
Sprache: | chi |
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Zusammenfassung: | Objective To determine the ELISA kit for screening convalescence plasma with high potency of SARS-CoV-2 IgG by comparing and analyzing the plasma detection results of convalescent plasma collected in different periods via ELISA kits from two manufacturers and the results of mixed plasma with different potency via pseudovirus neutralization experiments. Methods Two ELISA kits from different manufacturers(named A, B) were used to detect the plasma of 269 convalescent patients collected from Feb.2020~Jan.2022. The correlation and concordance rate of the two results were analyzed to determine the kit preliminarily. According to the titers of diluted series of standard of the preliminary selected kit, 5 mixed plasma samples (G4-G128) with different potency were prepared. The correlation of ELISA IgG results of product A/B, as well as the pseudovirus neutralization test of the original strain, Omicron mutant BA.1 and BA.2 strains were analyzed. Combined with the outside-well dilution mode of the strongly positive s |
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ISSN: | 1004-549X |
DOI: | 10.13303/j.cjbt.issn.1004-549x.2023.03.001 |