Histochemical study on distribution of mucopolysaccharides in trachea of buffalo during prenatal development

Histochemical study were made on trachea of buffalo foetuses  ranging from 4.3 to 44 cm CRL (crown rump length) to elucidate distribution of neutral and acid Mucopolysaccharides. During initial development up to 15.8 cm CRL tracheal eqithelium was strong to moderately positive from neutral mucopolysaccharides. The ground substance of developing cartilage primodria was weakly positive from acid mucopolysaccharide linging was present on the surface of PAS positive epithelia cells. The primordial of tracheal glands were positive for neutral mucopolysachharide content with well developed PAS positive submucosal tracheal glands.