Tailoring soy protein/corn zein mixture by limited enzymatic hydrolysis to improve digestibility and functionality

This study aimed to modify plant protein mixture to improve their functionality and digestibility by limited hydrolysis. Soy protein isolate and corn zein were mixed at the ratio of 5:1 (w/w), followed by limited hydrolysis using papain from 15 to 30 min. The structural characteristics, in vitro digestibility, and functional properties were evaluated. Also, DPPH radical scavenging activity was determined. The results indicated that the molecular weight of different modified samples was largely reduced by limited hydrolysis, and the proportion of random coil was significantly increased. Furthermore, the solubility, foaming, emulsifying and water-holding capacity of hydrolyzed protein mixture were significantly improved, which were close to those of whey protein isolate. In vitro digestibility after 30-min limited hydrolysis was remarkably elevated. In addition, the hydrolyzed protein mixture exhibited a higher antioxidant activity than those of untreated proteins. Overall, limited hydrolysis of protein mixture led to improved digestibility, functionality and antioxidant activity. Graphical abstract [Display omitted] •SPI-zein mixture with balanced essential amino acid composition was constructed.•Limited hydrolysis provoked a more disordered secondary structure.•Digestibility of protein mixture after limited hydrolysis was notably increased.•Limited hydrolysis led to significantly improved functional property.•DPPH radical scavenging activity was remarkably elevated by limited hydrolysis.