Bacterial Leakage Evaluation of Three Root Canal Sealers with Two Obturation Techniques: An in Vitro Study

Background: The purpose of this study was to compare the quality of the coronal seal of three root canal sealers and two obturation techniques using the bacterial penetration method. Methods and Results: A total of 132 single-rooted human teeth with fully developed apices were used. The teeth were r...

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Veröffentlicht in:International journal of biomedicine 2022-12, Vol.12 (4), p.584-590
Hauptverfasser: Kelmendi, Tringa, Dula, Linda, Kosumi, Shera, Kamberi, Anila, Kelmendi, Nita
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Sprache:eng
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Zusammenfassung:Background: The purpose of this study was to compare the quality of the coronal seal of three root canal sealers and two obturation techniques using the bacterial penetration method. Methods and Results: A total of 132 single-rooted human teeth with fully developed apices were used. The teeth were randomly assigned to three experimental groups according to the endodontic sealer used. Group 1: Samples (n=44) were obturated using a zinc oxide eugenol-based sealer, Pulp Canal Sealer EWT. Group 2: Samples (n=44) were obturated using an epoxy resin-based sealer, AH Plus. Group 3: Samples (n=44) were obturated using a bioceramic-based root canal sealer, Well-Root ST. Each group was subdivided into 2 equal subgroups in accordance with the obturation technique being used: CLCT and ThOT. Thus, 6 subgroups were formed: Sub-1A: Pulp Canal Sealer/CLCT; Sub-2A: AH Plus/CLCT; Sub-3A: Well-ROOT ST/CLCT; Sub-1B: Pulp Canal Sealer/ThOT; Sub-2B: Ah Plus/ThOT; Sub-3B: Well-ROOT ST/ThOT. A dual-chamber device was used to evaluate bacterial leakage. Fresh medium and E. faecalis were added to the upper chamber every 4 days. The broth was monitored for color change daily for 33 days. Significant differences were found among Sub-2A vs. Sub-1B (P=0.023), Sub-1A vs. Sub-3A (P=0.014), Sub-1A vs. Sub-2B (P=0.024), Sub-1A vs. Sub-3B (P=0.002), Sub-3A vs. Sub-1B (P=0.003), Sub-2B vs. Sub-1B (P=0.005), and Sub-1B vs. Sub-3B (P
ISSN:2158-0510
2158-0529
DOI:10.21103/Article12(4)_OA11