Exhaled breath SARS-CoV-2 shedding patterns across variants of concern
•We developed a silicon chip to capture exhaled breath (EB) aerosols and perform polymerase chain reaction (PCR).•Peak EB and nasopharynx (NP) SARS-CoV-2 shedding was comparable across variants.•EB qPCR positivity matches NP qPCR and supersedes NP rapid tests in early infection. We performed exhaled...
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Veröffentlicht in: | International journal of infectious diseases 2022-10, Vol.123, p.25-33 |
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Zusammenfassung: | •We developed a silicon chip to capture exhaled breath (EB) aerosols and perform polymerase chain reaction (PCR).•Peak EB and nasopharynx (NP) SARS-CoV-2 shedding was comparable across variants.•EB qPCR positivity matches NP qPCR and supersedes NP rapid tests in early infection.
We performed exhaled breath (EB) and nasopharyngeal (NP) quantitative polymerase chain reaction (qPCR) and NP rapid antigen testing (NP RAT) of SARS-CoV-2 infections with different variants.
We included immuno-naïve alpha-infected (n = 11) and partly boosted omicron-infected patients (n = 8) as high-risk contacts. We compared peak NP and EB qPCR cycle time (ct) values between cohorts (Wilcoxon-Mann-Whitney test). Test positivity was compared for three infection phases using Cochran Q test.
Peak median NP ct was 11.5 (interquartile range [IQR] 10.1-12.1) for alpha and 12.2 (IQR 11.1-15.3) for omicron infections. Peak median EB ct was 25.2 (IQR 24.5-26.9) and 28.3 (IQR 26.4-30.8) for alpha and omicron infections, respectively. Distributions did not differ between cohorts for NP (P = 0.19) or EB (P = 0.09). SARS-CoV-2 shedding peaked on day 1 in EB (confidence interval [CI] 0.0 - 4.5) and day 3 in NP (CI 1.5 - 6.0). EB qPCR positivity equaled NP qPCR positivity on D0-D1 (P = 0.44) and D2-D6 (P = 1.0). It superseded NP RAT positivity on D0-D1 (P = 0.003) and D2-D6 (P = 0.008). It was inferior to both on D7-D10 (P < 0.001).
Peak EB and nasopharynx shedding were comparable across variants. EB qPCR positivity matched NP qPCR and superseded NP RAT in the first week of infection. |
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ISSN: | 1201-9712 1878-3511 1878-3511 |
DOI: | 10.1016/j.ijid.2022.07.069 |