Use of Tetra-ammonium Tetrakis(4-Sulphonato)Phenyl Porphyrin for Pseudomonas and Bacillus Cell Imaging

Use of Tetra-ammonium Tetrakis(4-Sulphonato)Phenyl Porphyrin for Pseudomonas and Bacillus Cell Imaging

The use of tetraammonium tetrakis(4-sulphonato)phenyl porphyrin (TPPS), a water-soluble anionic compound, as a stain to analyse bacterial cells using fluorescent microscopy was investigated. TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus. The...

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Veröffentlicht in:International Journal of Analytical Chemistry 2010-01, Vol.2010 (2010), p.98-100
Hauptverfasser: Sridhar, Bharat, Sujatha, V., Krishnamurthy, Srinath, Vinod Kumar, K. S., Senthil Kumar, K., Gautam, Pennathur
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Bacillus
Bacillus cereus
Bacteria
Gram-negative bacteria
Mass spectrometry
Microbiology
Pseudomonas aeruginosa
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Zusammenfassung:The use of tetraammonium tetrakis(4-sulphonato)phenyl porphyrin (TPPS), a water-soluble anionic compound, as a stain to analyse bacterial cells using fluorescent microscopy was investigated. TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus. The variation in brightness with varying concentrations of TPPS was studied. The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.
ISSN:1687-8760
1687-8779
DOI:10.1155/2010/697528