Up-regulation expression of protein kinase D2 reduces lung ischemia-reperfusion injury in mice

Objective To observe the effect of up-regulation of protein kinase D2 (PKD2) expression on lung ischemia-reperfusion injury (LI/RI) in mice. Methods 20 C57BL/6J wild-type (WT) mice and 40 PKD2 over-expression (PKD2+/+) mice were randomly divided into sham operation group (WS and PS groups), LI/RI group (WIR and PIR groups, the left hilum was first clamped for 1 h, then reperfused for 2 h) and the hypoxia inducible factor-1α (HIF-1α) inhibitor (2-methoxyestradiol, 2-ME) group (WIR+2ME) and (PIR+2ME) groups, 20 mg/(kg·d) 2ME was injected intraperitoneally 2 days before modeling). After 2 hours of reperfusion, the wet/dry weight ratio(W/D) of lung tissues was detected, the pathological changes of lung tissues were observed by HE staining, and the content of inflammatory factors TNF-α, IL-1β and IL-6 in lung tissues were detected by ELISA. RT-qPCR and Western blot were used to detect the expression of (HIF-1α) and vascular endothelial growth factor A(VEGFA) mRNA and protein in lung tissues. Results Compared with