Personalized early diagnosis and prediction of the tuberculosis infection course in children identifying predictors of latent tuberculosis infection and tuberculosis

The objective: to determine predictors of the development of latent tuberculosis infection (LTBI) and tuberculosis in children and adolescents.Subjects and methods. A study was carried out, including testing induced IFN-y and assessment of genotypes of the polymorphic variant of IFNG gene (T-1488C) among 310 children under the age of 18. They included children ill with tuberculosis, infected with tuberculous mycobacteria (MTB) and not infected with MTB.Results. It was found that the main predictors of LTBI development were biomedical factors, for LTBI progression - social factors. The marker of the high risk to develop tuberculosis (OR = 4.667, 95% CI 1.24-17.62; p = 0.008) for both primary (47.5%) and secondary (65.0%) genesis of the disease and its unfavorable course is the heterozygous IFNG genotype (T-1488C). The probable risk of tuberculosis progression in this variant is found to be at the level of 74.07% (95% CI 63.54-82.43%).At the early stage, LTBI markers were specific proteins: ESAT6, Rv2660c. The ESAT6-CFP10 hybrid protein was identified as a marker of active tuberculosis infection.