Prevalence and risk factors for HPV seropositivity and anogenital DNA positivity among men who have sex with men: a repeated cross-sectional study

•Human papillomavirus (HPV) seropositivity and anogenital HPV deoxyribonucleic acid (DNA)-positivity are common among young men who have sex with men (MSM).•Receptive anal intercourse (RAI) increases HPV seroprevalence and anal prevalence.•MSM with RAI generally have higher median HPV antibody titers.•Anal HPV might be more likely to lead to seroconversion than penile HPV for MSM. This study aimed to assess associations of potential risk factors with human papillomavirus (HPV) seropositivity among men who have sex with men (MSM) and compare these to risk factors for anal and penile (HPV) deoxyribonucleic acid (DNA)-positivity in the same study population. Seropositivity and anal and penile HPV DNA-positivity were determined for seven high-risk HPV genotypes for MSM aged 16-24 years participating in Papillomavirus Surveillance among STI clinic Youngsters in the Netherlands (PASSYON) 2009-2021. Logistic regression models were conducted to assess risk factors for seropositivity, anal and penile HPV DNA-positivity. Overall, 1019 MSM were included. HPV-16 and -18 were most common for serology, and anal and penile HPV DNA-positivity. Although no clear similarities were observed for most risk factors for HPV seropositivity and anal or penile DNA positivity, receptive anal intercourse (RAI) was the strongest associated risk factor for both seropositivity (‘RAI ever’ adjusted odds ratio [aOR] 3.50, 95% confidence interval [CI] 1.56-7.88; ‘RAI previous 6 months’ aOR 2.17, 95% CI 1.44-3.26) and anal DNA-positivity (‘RAI previous 6 months’ aOR 1.67, 95% CI 1.09-2.56). Our study is suggestive of site-specific immune response after HPV infection; RAI might lead to anal HPV infections and consequently to seroconversion. Finally, as the two genotypes that are most oncogenic and preventable by all HPV vaccines were most common, our results underline the importance of gender-neutral vaccination.