Effect of chromium citrate on lipid composition in blood plasma of rats with experimental diabetes

The results of studying changes of lipid composition in blood plasma of rats with alloxan-induced diabetes under the condition of consumption of chromium citrate in the concentration of 0.1 and 0.2 µg/ml of water are presented. The experiment was conducted on 32 laboratory rats. The rats were divided in four groups: K1 – control group, K2 – control group with diabetes, R1 and R2 – experimental groups. Pure water (without any additives) was given to the rats of groups K1 and K2. Water with chromium citrate in concentration of 0.1 and 0.2 µg/ml was given to the animals from groups R1 and R2 for one month. Experimental diabetes mellitus was induced in the animals of groups K2, R1, and R2 by the intraperitoneal injection of 5% solution of alloxan monohydrate in concentration of 150 mg/kg of body weight. Total lipids content and their classes were determined in animals’ blood plasma. It was found that an imbalance was present in the lipid profile and the phospholipid profile of animals with experimental diabetes mellitus. In particular, the content of total lipids and relative content of non-esterified cholesterol increased significantly, and there was a tendency of the content of triacylglycerols and unesterified fatty acids to be increased in the rats’ blood with experimental diabetes mellitus of K2 group. There was a shift in spectrum of different fractions when the content of phospholipids decreased in blood of rats with experimental diabetes mellitus. The content of total lipids, esterified and non-esterified cholesterol significantly decreased and the content of triacylglycerols and unesterified fatty acids was significantly lower in animals of the experimental groups R1 and R2, but the content of phospholipids increased in the same groups. Changes in content of certain classes of lipids in blood of rats with alloxan diabetes affected by additives of citrate chromium are multidirectional and dose-dependent.