Effect of epidermal growth factor on buffalo frozen spermatozoa biometry and metabolic activity

Effect of epidermal growth factor on buffalo frozen spermatozoa biometry and metabolic activity

Objective:To assess the effects of epidermal growth factor (EGF) on the Egyptian buffalo bull frozen semen, EGF was incorporated at 0 (control), 50, 100, 200 and 400 ng/mL of extender (Bioxcell?).Methods:Semen features, spermatozoa biometry, total liberated amounts of enzymes (aspartate transaminase...

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Veröffentlicht in:Asian Pacific Journal of Reproduction 2017-01, Vol.6 (1), p.43-48
Hauptverfasser: Kandiel, Mohamed, El-Khawagah, Ahmed, Mahmoud, Karima
Format: Artikel
Sprache:eng
Schlagworte:
Amino acids
Aspartate
Biometry
Buffalo
DNA
Epidermal growth factor
Epidermal growth factors
Lipid peroxidation
Nitric oxide
Nitrogen oxides
Peroxidase
Phosphatases
Seminal enzymes
Speriogram
Sperm
Superoxides
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Zusammenfassung:Objective:To assess the effects of epidermal growth factor (EGF) on the Egyptian buffalo bull frozen semen, EGF was incorporated at 0 (control), 50, 100, 200 and 400 ng/mL of extender (Bioxcell?).Methods:Semen features, spermatozoa biometry, total liberated amounts of enzymes (aspartate transaminase, alanine aminotransferase, lactate dehydrogenase, acid phosphatase and alkaline phosphatase) and lipid peroxidation markers (thiobarbituric acid reactive substances, malondialdehyde, glutathione peroxidase, nitric oxide, catalase (CAT) and superoxide dismutase (SOD)) were determined in the spermatozoa-free extracellular extender. Results:Spermatozoa membrane integrity significantly (P
ISSN:2305-0500
2305-0519
DOI:10.12980/apjr.6.20170108