The GP-45 Protein, a Highly Variable Antigen from Babesia bigemina , Contains Conserved B-Cell Epitopes in Geographically Distant Isolates

In , the 45 kilodaltons glycoprotein (GP-45) is the most studied. GP-45 is exposed on the surface of the merozoite, it is believed to play a role in the invasion of erythrocytes, and it is characterized by a high genetic and antigenic polymorphism. The objective of this study was to determine if GP-...

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Veröffentlicht in:Pathogens (Basel) 2022-05, Vol.11 (5), p.591
Hauptverfasser: Mercado-Uriostegui, Miguel Angel, Castro-Sánchez, Luis Alberto, Batiha, Gaber El-Saber, Valdez-Espinoza, Uriel Mauricio, Falcón-Neri, Alfonso, Ramos-Aragon, Juan Alberto, Hernández-Ortiz, Ruben, Kawazu, Shin-Ichiro, Igarashi, Ikuo, Mosqueda, Juan
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Sprache:eng
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Zusammenfassung:In , the 45 kilodaltons glycoprotein (GP-45) is the most studied. GP-45 is exposed on the surface of the merozoite, it is believed to play a role in the invasion of erythrocytes, and it is characterized by a high genetic and antigenic polymorphism. The objective of this study was to determine if GP-45 contains conserved B-cell epitopes, and if they would induce neutralizing antibodies. The comparative analysis of nucleotide and amino acids sequences revealed a high percentage of similarity between field isolates. Antibodies against peptides containing conserved B-cell epitopes of GP-45 were generated. Antibodies present in the sera of mice immunized with GP-45 peptides specifically recognize by the IFAT. More than 95% of cattle naturally infected with contained antibodies against conserved GP-45 peptides tested by ELISA. Finally, sera from rabbits immunized with GP-45 peptides were evaluated in vitro neutralization tests and it was shown that they reduced the percentage of parasitemia compared to sera from rabbits immunized with adjuvant. GP-45 from geographically distant isolates of contains conserved B-cell epitopes that induce neutralizing antibodies suggesting that this gene and its product play a critical role in the survival of the parasite under field conditions.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens11050591