Limited antibody specificity compromises epitranscriptomic analyses

A controversial discussion on the occurrence of the RNA modification m 1 A in mRNA takes a new turn, as an antibody with a central role in modification mapping was shown to also bind mRNA cap structures.

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Bibliographische Detailangaben
Veröffentlicht in:	Nature communications 2019-12, Vol.10 (1), p.5669-3, Article 5669
Hauptverfasser:	Helm, Mark, Lyko, Frank, Motorin, Yuri
Format:	Artikel
Sprache:	eng
Schlagworte:	38/1 38/90 38/91 631/337/1645/2570 631/45/500 Animals Antibodies Antibodies - genetics Antibodies - metabolism Antibody Specificity Biochemistry, Molecular Biology Comment DNA Methylation Epigenesis, Genetic Epigenetics Experiments Gene expression Gene Expression Regulation Genomes Genomics Humanities and Social Sciences Humans Life Sciences Molecular biology mRNA multidisciplinary Peptides Protein Binding RNA modification RNA, Messenger - genetics RNA, Messenger - metabolism Science Science (multidisciplinary) Transcriptome
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Beschreibung
Zusammenfassung:	A controversial discussion on the occurrence of the RNA modification m 1 A in mRNA takes a new turn, as an antibody with a central role in modification mapping was shown to also bind mRNA cap structures.
ISSN:	2041-1723 2041-1723
DOI:	10.1038/s41467-019-13684-3