Double Sandwich ELISA Modified Method for the Detection of Clostridium Botulinum Type E

Background & Objective: A very small amount of botulinum toxin can cause death and on the other hand, there is no cure for its poison other than antitoxin. Therefore, a diagnostic method that can detect very small amounts of botulinum toxin in a short time is very important. In this study, rapid and accurate detection of botulinum toxin type E has been performed with the double sandwich ELISA method. Materials & Methods: based on the double sandwich ELISA method two different antibodies that bind to epitopes without the interference of antigens is required. For this purpose, the rabbit antibody was poured at the bottom (Capture antibody) and mouse antibody at the top of the toxin (Detection antibody) of each well and the toxin was titrated from the first wells. Then, the modified sandwich ELISA system was set up and optimized for the detection of botulinum toxin type E.Results: The modified double sandwich ELISA method is able to detect Clostridium botulinum type E toxin to a value of 1 ng in less than 6 hours. Conclusion: The modified double sandwich ELISA method is very precise and fast in detecting Clostridium botulinum type E. This method is used for determining specific antigen in the unknown sample.