Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile tilapia, Oreochromis niloticus (Linnaeus, 1757)

A hundred adult of tilapia, Oreochromis niloticus, were inoculated intraperitoneally with 10(8) c.f.u. of Mycobacterium marinum. Other 100 speciemns were maintained without inoculation. Blood samples of six inoculated animals were collected at 01, 03, 07, 14, 21, 28, 35, 42, 49, 56 and 84 days after...

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Veröffentlicht in:Brazilian archives of biology and technology 2004-11, Vol.47 (6), p.945-953
Hauptverfasser: Ranzani-Paiva, Maria José Tavares, Ishikawa, Carlos Massatoshi, Eiras, Augusta Cocuzza das, Silveira, Vivian Risaffi da
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Sprache:eng
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Zusammenfassung:A hundred adult of tilapia, Oreochromis niloticus, were inoculated intraperitoneally with 10(8) c.f.u. of Mycobacterium marinum. Other 100 speciemns were maintained without inoculation. Blood samples of six inoculated animals were collected at 01, 03, 07, 14, 21, 28, 35, 42, 49, 56 and 84 days after inoculation. Initially, six specimens were taken from the non inoculated group ("time zero"). The haematological parameters determined were: haematocrit (Hct), haemoglobin concentration (Hgb), total erythrocytes count (RBC), hematological indexes (MCV, MCH and MCHC), thrombocyte count (Tr), leukocyte count (WBC) and leukocyte differential count. There were no changes in Hct, Hgb, MCH and MCHC. However, the mean values for RBC and MCV decreased. The mean values for RBC and Hct of specimens from the "time zero" group were higher and the MCV and MCH lower than in inoculated animals. Occurred neutrophilia, and, at 72 h there was lymphocytosis and neutropenia. By the 49th day after bacterial inoculation, there was monocytosis. Cem exemplares adultos de tilápia, Oreochromis niloticus, foram inoculadas intraperitonealmente com 10(8) UFC de Mycobacterium marinum e outras 100 foram mantidas sem inoculação. Inicialmente, o sangue de 6 exemplares não inoculados foi colhido e considerado como "tempo zero". Do grupo dos inoculados foi retirado sangue de 6 animais por colheita, 1, 3, 7, 14, 21, 28, 35, 42, 49, 56 e 84 dias após inoculação. Os parâmetros hematológicos: hematócrito (Hct), taxa de hemoglobina (Hgb), contagem de eritrócitos (RBC), foram determinados e os índices hematológicos (VCM, HCM e CHCM), calculados. Foram determinados também o número de trombócitos e o número total e diferencial de leucócitos. Não ocorreram alterações nos valores de Hct, Hgb, HCM e CHCM. Entretanto houve diminuição dos valores de RBC e VCM. Os valores médios de RBC e Hct dos animais no "tempo zero" foram maiores e os de VCM e HCM, menores que os dos animais inoculados. Ocorreu neutrofilia e, após 3 dias de inoculação, ocorreram linfocitose e neutropenia nos animais. No 49º. dia após inoculação bacteriana, ocorreu monocitose.
ISSN:1516-8913
1516-8913
1678-4324
DOI:10.1590/S1516-89132004000600014