Characteristics of Water-soluble Pectin from Greengage and Its Anti-inflammatory Activity in LPS-induced RAW246.7 Cells

In this study, the water soluble pectin (WSP) from greengage juice was extracted. The structure and composition of WSP were characterized by high performance liquid chromatography (HPLC), high performance gel filtration chromatography (HPGFC), Fourier transform infrared spectrometer (FT-IR), scanning electron microscope (SEM) and X-ray diffraction (XRD). And the anti-inflammatory activity of WSP in lipopolysaccharide (LPS)-induced RAW246.7 cells was evaluated by enzyme-linked immunosorbent assay (ELISA). The results showed that WSP was a low-methoxy pectin with DE value of 18.89%. The content of galacturonic acid (GalA) was 69.72%, and the galactose (Gal) was the predominant neutral sugar with the content of 18.36%. The molecular weight (Mw) of WSP was 353.73 kDa. WSP significantly inhibited the release of tumor necrosis factor α (TNF-α) (P