Rab4b Promotes Cytolethal Distending Toxin from Glaesserella parasuis -Induced Cytotoxicity in PK-15 Cells

cytolethal distending toxin ( CDT) can induce cell cycle arrest and apoptosis. Our laboratory's previous work demonstrated that GTPase 4b (Rab4b) is a key host protein implicated in CDT-induced cytotoxicity. This study investigated the probable involvement of Rab4b in the process. Our study use...

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Veröffentlicht in:Toxins 2024-09, Vol.16 (9), p.407
Hauptverfasser: Zhang, Yiwen, Yang, Zhen, Dai, Ke, Hu, Bangdi, Xu, Shiyu, Wang, Yu, Lei, Li, Du, Senyan, Zhao, Qin, Huang, Xiaobo, Wu, Rui, Yan, Qigui, Wang, Yiping, Cao, Sanjie, Wen, Yiping
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Sprache:eng
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Zusammenfassung:cytolethal distending toxin ( CDT) can induce cell cycle arrest and apoptosis. Our laboratory's previous work demonstrated that GTPase 4b (Rab4b) is a key host protein implicated in CDT-induced cytotoxicity. This study investigated the probable involvement of Rab4b in the process. Our study used CRISPR/Cas9 technology to create a Rab4b-knockout cell line. The results showed greater resistance to CDT-induced cell cytotoxicity. In contrast, forced Rab4b overexpression increased CDT-induced cytotoxicity. Further immunoprecipitation study reveals that CDT may bind with Rab4b. In PK-15 cells, CDT is transported to the early endosomes and late endosomes, while after knocking out Rab4b, CDT cannot be transported to the early endosome via vesicles. Rab4b appears essential for CDT-induced cytotoxicity in PK-15 cells.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins16090407