Rab4b Promotes Cytolethal Distending Toxin from Glaesserella parasuis -Induced Cytotoxicity in PK-15 Cells
cytolethal distending toxin ( CDT) can induce cell cycle arrest and apoptosis. Our laboratory's previous work demonstrated that GTPase 4b (Rab4b) is a key host protein implicated in CDT-induced cytotoxicity. This study investigated the probable involvement of Rab4b in the process. Our study use...
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Veröffentlicht in: | Toxins 2024-09, Vol.16 (9), p.407 |
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Sprache: | eng |
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Zusammenfassung: | cytolethal distending toxin (
CDT) can induce cell cycle arrest and apoptosis. Our laboratory's previous work demonstrated that GTPase 4b (Rab4b) is a key host protein implicated in
CDT-induced cytotoxicity. This study investigated the probable involvement of Rab4b in the process. Our study used CRISPR/Cas9 technology to create a Rab4b-knockout cell line. The results showed greater resistance to
CDT-induced cell cytotoxicity. In contrast, forced Rab4b overexpression increased
CDT-induced cytotoxicity. Further immunoprecipitation study reveals that
CDT may bind with Rab4b. In PK-15 cells,
CDT is transported to the early endosomes and late endosomes, while after knocking out Rab4b,
CDT cannot be transported to the early endosome via vesicles. Rab4b appears essential for
CDT-induced cytotoxicity in PK-15 cells. |
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ISSN: | 2072-6651 2072-6651 |
DOI: | 10.3390/toxins16090407 |