Single step BP/LR combined Gateway reactions

The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	BioTechniques 2013-11, Vol.55 (5), p.265-268
Hauptverfasser:	Liang, Xiquan, Peng, Lansha, Baek, Chang-Ho, Katzen, Federico
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda - genetics clonase cloning Cloning, Molecular - methods DNA - genetics Genetic Vectors - genetics one-pot reaction Plasmids - genetics recombination Recombination, Genetic
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we report reaction conditions for cloning DNA fragments into destination vectors in a single step reaction, thus reducing the cost and overall time needed to obtain an expression clone from three days to one.
ISSN:	0736-6205 1940-9818
DOI:	10.2144/000114101