Temporal heterogeneity in photosystem II photochemistry in Artemisia ordosica under a fluctuating desert environment

Acclimation strategies in xerophytic plants to stressed environmental conditions vary with temporal scales. Our understanding of environmentally-induced variation in photosystem II (PSII) processes as a function of temporal scales is limited, as most studies have thus far been based on short-term, laboratory-controlled experiments. In a study of PSII processes, we acquired near-continuous, field-based measurements of PSII-energy partitioning in a dominant desert-shrub species, namely Artemisia ordosica , over a six-year period from 2012–2017. Continuous-wavelet transformation (CWT) and wavelet coherence analyses (WTC) were employed to examine the role of environmental variables in controlling the variation in the three main PSII-energy allocation pathways, i.e., photochemical efficiency and regulated and non-regulated thermal dissipation, i.e., Φ PSII , Φ NPQ , and Φ NO , respectively, across a time-frequency domain from hours to years. Convergent cross mapping (CCM) was subsequently used to isolate cause-and-effect interactions in PSII-energy partitioning response. The CWT method revealed that the three PSII-energy allocation pathways all had distinct daily periodicities, oscillating abruptly at intermediate timescales from days to weeks. On a diurnal scale, WTC revealed that all three pathways were influenced by photosynthetically active radiation ( PAR ), air temperature ( T a ), and vapor pressure deficit ( VPD ). By comparing associated time lags for the three forms of energy partitioning at diurnal scales, revealed that the sensitivity of response was more acutely influenced by PAR , declining thereafter with the other environmental variables, such that the order of influence was greatest for T a , followed by VPD , and then soil water content ( SWC) . PSII-energy partitioning on a seasonal scale, in contrast, displayed greater variability among the different environmental variables, e.g., Φ PSII and Φ NO being more predisposed to changes in T a , and Φ NPQ to changes in VPD . CCM confirmed the causal relationship between pairings of PSII-energy allocation pathways, according to shrub phenology. A. ordosica is shown to have an innate ability to (i) repair damaged PSII-photochemical apparatus (maximum quantum yield of PSII photochemistry, with F v / F m > 0.78), and (ii) acclimatize to excessive PAR , dry-air conditions, and prolonged drought. A. ordosica is relatively sensitive to extreme temperature and exhibits photoinhibition.