The effect of Ca-Dobesilate over renal scar formation in an experimental pyelonephritis model

Purpose: This study was conducted to evaluate the effects of the drug: Ca-Dobesilate (CaD) which has been in common use in venous insufficiency treatment; on renal scarring and expressions of transforming growth factor beta1 (TGFb1), basic fibroblast growth factor (bFGF) and hepatocyte growth factor-beta (HGF-beta) in a rat pyelonephritis model. Materialw and Methods: Eight pyelonephritis groups, each constituting of 7 rats were developed as no treatment - ciprofloxacin – ciprofloxacin and CaD administered groups; following injecting E Coli (ATCC 25922) into kidney. No treatment given rat groups were sacrificed following 24h, 72 h, 14d and 28d from bacterial seeding respectively. Rats from treatment groups were sacrificed after 14d and 28d accordingly. Diagnoses of pyelonephritis and fibrosis, TGFb, bFGF and HGF-beta were scored semiquantitatively by immunohistochemical staining. Results: The extent of pyelonephritis and fibrosis was lower in rats treated with ciprofloxacin and CaD compared to sole ciprofloxacin and no treatment administered counterparts among groups terminated after 2wks following bacterial inoculation. However, CaD effect on pyelonephritis and fibrosis scores did not persist after treatment was discontinued. Conclusion: CaD might alleviate pyelonephritis and scarring, depending on dosage and treatment period and further studies are needed to determine optimum treatment dose and duration. Amaç: Bu çalışma, venöz yetmezlikte yaygın kullanımda olan kalsiyum dobesilatın, sıçan piyelonefrit modelinde renal skar ve transforming büyüme faktörü beta1 (TGFb1), temel fibroblast büyüme faktörü (bFGF) ve hepatosit büyüme faktörü-beta (HGF-beta) dokudaki düzeylerini değerlendirmeyi amaçlamıştır. Gereç ve Yöntem: Böbreğe E Coli (ATCC 25922) enjeksiyonunu takiben; tedavisiz- siprofloksasin alan – siprofloksasin ve kalsiyum dobesilat uygulanan olmak üzere her biri 7 sıçandan oluşan gruplar oluşturuldu. Tedavi almayan sıçan grupları bakteri ekimini takiben; sırası ile 24 ve 72 saat sonra ve 14 ve 28 gün sonra sonlandırıldı. Tedavi alan gruplardaki sıçanlar ise 14 ve 28 gün sonra sonlandırıldılar. Piyelonefrit ve fibrosis tanıları, TGFb, bFGF, HGF-beta semikantitatif olarak immünhistokimya boyama ile skorlandı. Bulgular: Bakteriyel inokulasyondan 14 gün sonra sonuçlandırılan gruplar içinde; siprofloksasin ve kalsiyum dobesilat birlikte uygulanan sıçanlarda sadece siprofloksasin alan ve hiç tedavi almayan sıçanlara göre piyelonefrit ve fibrosis yayılımı da