Functional and mechanistic studies of a phytogenic formulation, Shrimp Best, in growth performance and vibriosis in whiteleg shrimp
Climate change and disease threaten shrimp farming. Here, we studied the beneficial properties of a phytogenic formulation, Shrimp Best (SB), in whiteleg shrimp. Functional studies showed that SB dose-dependently increased shrimp body weight and decreased feed conversion ratio. We found that SB prot...
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Veröffentlicht in: | Scientific reports 2024-05, Vol.14 (1), p.11584-11584, Article 11584 |
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Sprache: | eng |
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Zusammenfassung: | Climate change and disease threaten shrimp farming. Here, we studied the beneficial properties of a phytogenic formulation, Shrimp Best (SB), in whiteleg shrimp. Functional studies showed that SB dose-dependently increased shrimp body weight and decreased feed conversion ratio. We found that SB protected against
Vibrio parahaemolyticus
as evidenced by survival rate, bacterial load, and hepatopancreatic pathology in shrimp. Finally, we explored the likely mechanism by which SB affects growth performance and vibriosis in shrimp. The 16S rRNA sequencing data showed that SB increased 6 probiotic genera and decreased 6 genera of pathogenic bacteria in shrimp. Among these, SB increased the proportion of
Lactobacillus johnsonii
and decreased that of
V. parahaemolyticus
in shrimp guts. To dissect the relationship among SB,
Lactobacillus
and
Vibrio
, we investigated the in vitro regulation of
Lactobacillus
and
Vibrio
by SB. SB at ≥ 0.25 μg/mL promoted
L. johnsonii
growth. Additionally,
L. johnsonii
and its supernatant could inhibit
V. parahaemolyticus
. Furthermore, SB could up-regulate five anti-
Vibrio
metabolites of
L. johnsonii
, which caused bacterial membrane destruction. In parallel, we identified 3 fatty acids as active compounds from SB. Overall, this work demonstrated that SB improved growth performance and vibriosis protection in shrimp via the regulation of gut microbiota. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-024-62436-x |