Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing

Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing

Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyot...

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Veröffentlicht in:Stem cell research 2018-05, Vol.29, p.215-219
Hauptverfasser: Xu, An, Zhou, Ruoji, Tu, Jian, Huo, Zijun, Zhu, Dandan, Wang, Donghui, Gingold, Julian A., Mata, Helen, Rao, Pulivarthi H., Liu, Mo, Mohamed, Alaa M.T., Kong, Celine Shuet Lin, Jewell, Brittany E., Xia, Weiya, Zhao, Ruiying, Hung, Mien-Chie, Lee, Dung-Fang
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Sprache:eng
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Zusammenfassung:Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2018.04.013