Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lytic...

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Veröffentlicht in:Iranian journal of microbiology 2012-03, Vol.4 (1), p.44-46
Hauptverfasser: Ghodousi, Arash, Arash, Ghodousi A, Vatani, S, Darban-Sarokhalil, Davood, Omrani, Maryam, Fooladi, A, Fooladi, Aa, Khosaravi, A, Khosaravi, Ad, Feizabadi, Mohammad Mehdi
Format: Artikel
Sprache:eng
Schlagworte:
Mycobacterium tuberculosis
Original
Pulsed-filed gel electrophoresis
TB-complex
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Zusammenfassung:A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.
ISSN:2008-3289
2008-4447