The anti-parasitic effect of probiotic bacteria via limiting the fecundity of Trichinella spiralis female adults

A potential protective effect of probiotic strains against zoonotic infection was investigated in the framework of a new therapeutic strategy aimed at using probiotics to control parasitic zoonoses. The study was focused on the impact of six selected probiotic (bacteriocinogenic) strains on the intensity of . infection and female fecundity and . Bacterial strains of different origin ( EF55, 2019 = CCM7420, AL41 = CCM8558, ED26E/7, AD1 = CCM7421, 17L/1) were administered daily in a dose of 10 CFU/ml in 100 μl, and mice were infected with 400 . larvae on day 7 of treatment. Female adults of . were isolated on day 5 post infection (p.i.) and subsequently were used in fecundity test . . CCM8558, . CCM7420 and . ED26E/7 strains significantly reduced the number of adults in the intestine. The application of . CCM7421, . 17L/1, . CCM8558 and . ED26E/7 caused a significant decrease in the number of muscle larvae. The treatment with . CCM8558 and . ED26E/7 showed the highest inhibitory effect on female fecundity (94 %). The number of newborn larvae (NBL) was also significantly decreased after administration of . CCM7421 and . 17L/1 (80 %). A direct impact of probiotic strains on female reproductive capacity was examined in females isolated from untreated infected mice on day 5 p.i. A correlation was found between the inhibitory effect and the concentration of probiotic strains. The reduction effects of the strains manifested as follows: . CCM7421 (93 %), . CCM8558, . 17L/1, . EF55 (about 80 %), . CCM7420 and . ED26E/7 (about 60 %).